This Dataverse contains datasets generated and published by the Greathouse Lab, housed within Baylor University. Our research focuses on the intersection of the gut microbiome, nutrition, host immunity, and cancer, with a particular emphasis on Fusobacterium nucleatum and its role in colorectal cancer (CRC) pathogenesis. The datasets stored here include raw and processed data from microbiome sequencing, metabolite profiling (e.g., indole derivatives), gene expression analyses, biofilm formation assays, and associated computational code used in peer-reviewed publications. All data are curated to support transparency, reproducibility, and data reuse in microbiome and cancer research. This repository serves as an open-access resource for researchers investigating microbe-host interactions, microbial metabolism, and cancer-associated microbial ecology.