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Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a
Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a
May 19, 2025
Russell, Rick, 2025, "Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a", https://doi.org/10.18738/T8/P6HTGJ, Texas Data Repository, V5
CRISPR-Cas12a binds and processes a single pre-crRNA during maturation, providing a simple tool for genome editing applications. Here, we constructed a kinetic and thermodynamic framework for pre-crRNA processing by Cas12a in vitro, and we measured the contributions of distinct r...
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